Journal: BMC Cancer

Article Title: The role of S100A4 for bone metastasis in prostate cancer cells

doi: 10.1186/s12885-021-07850-4

Figure Lengend Snippet: S100A4 expression and secretion is elevated in bone-metastatic prostate cancer cells. a Flow cytometry with anti-human β2-microglobulin Ab verified these bone-metastatic PC3 cells (mtPC3s) as human cells. Neg. Cont., mouse bone marrow cells; Pos. Cont., HEK-293 cells. b S100A4 mRNA expression in LNCaP, PC3, and mtPC3 cells was analyzed with real-time PCR. n = 3 per group. c S100A4 protein expression in LNCaP, PC3, and mtPC3 cells was analyzed by western-blotting. d Levels of S100A4 protein secretion in LNCaP, PC3, and mtPC3 cells in cell culture medium were measured by ELISA. n = 3 per group. *** p < 0.001 by one-way ANOVA with post hoc Tukey’s test. Data are presented as the mean ± SD. The full-length gels are presented in Supplementary Fig. a

Article Snippet: mtPC3 cells were authenticated by labelling with PE-conjugated mouse anti-human β2-microglobulin (BD Pharmingen).

Techniques: Expressing, Flow Cytometry, Real-time Polymerase Chain Reaction, Western Blot, Cell Culture, Enzyme-linked Immunosorbent Assay

Journal:

Article Title: Of the three tegument proteins that package mRNA in herpes simplex virions, one (VP22) transports the mRNA to uninfected cells for expression prior to viral infection

doi: 10.1073/pnas.122231699

Figure Lengend Snippet: Two-color flow cytometry analyses for simultaneous detection of chimeric US8.5-EGFP (FL1) protein and β2 microglobulin (FL2) in Vero and/or HEp-2 cells. (A) Untransduced Vero cells stained with anti-human β2 microglobulin. (B) Vero cells transduced with 10 pfu of Bac-US8.5-EGFP and reacted with anti-human β2 microglobulin. (C) Untransduced HEp-2 cells stained with anti-human β2 microglobulin. (D) HEp-2 cells transduced with 10 pfu of Bac-US8.5-EGFP and reacted with anti-human β2 microglobulin. (E) HEp-2 cells transduced with 10 pfu of Bac-US8.5-EGFP, cocultivated with untreated Vero cells, and then reacted with anti-human β2 microglobulin. (F) HEp-2 cells transduced with a mixture of 25 pfu of Bac-UL49 plus 10 pfu of Bac-US8.5-EGFP, cocultivated with untreated Vero cells, and then reacted with anti-human β2 microglobulin. (G) HEp-2 cells transduced and cocultivated with untreated Vero cells as in F but treated by the addition of RNase to the medium during the cocultivation.

Article Snippet: To analyze the simultaneous expression of EGFP (FL1, green) and a surface marker (FL2, red), the cells were reacted with a monoclonal antibody against human β 2 microglobulin (Becton Dickinson) for 30 min on ice and rinsed twice in PBS, reacted with a R-phycoerythrin-conjugated goat anti-mouse IgG (Molecular Probes), fixed with a paraformaldeide solution, and analyzed on a FACScan (Becton Dickinson) by using cell-quest software.

Techniques: Flow Cytometry, Staining, Transduction